2 edition of Nucleotide sequence of two chloroplast genes from a Chlorella-like green alga found in the catalog.
Nucleotide sequence of two chloroplast genes from a Chlorella-like green alga
Sean M. Amberg
Written in English
|Statement||by Sean M. Amberg.|
|The Physical Object|
|Pagination||23 leaves, bound :|
|Number of Pages||23|
A stem-loop region is present at the 3′ terminus of the chloroplast rbcL mRNA in all taxa surveyed to date. In spinach, this structure has been shown . Metabolism, cell cycle stages, and related transcriptomes in eukaryotic algae change with the diel cycle of light availability. In the unicellular red alga Cyanidioschyzon merolae, the S and M phases occur at night. To examine how diel transcriptomic changes in metabolic pathways are related to the cell cycle and to identify all genes for which mRNA levels change depending on the cell cycle.
To study the role of initiation codon context in chloroplast protein synthesis, we mutated the three nucleotides immediately upstream of the initiation codon (the −1 triplet) of two chloroplast genes in the alga Chlamydomonas reinhardtii. In prokaryotes, the −1 triplet has been proposed to base pair with either the loop of 16S rRNA or the extended anticodon of fMet-tRNA. It should be noted that the chloroplast DNA of P. purpurea also contains two repeats encompassing rRNA genes; however, unlike in green algal and plant chloroplast DNAs, these repeats are arranged in direct orientation, differ from one another in sequence, and do not appear to support intramolecular recombination (Reith and Munholland, b).
Often during flowering plant evolution, ribosomal protein genes have been lost from the mitochondrion and transferred to the nucleus. Here, we show that substitution by a duplicated, divergent gene originally encoding the chloroplast or cytosolic ribosomal protein counterpart accounts for two missing mitochondrial genes in diverse angiosperms. The rps13 gene is missing from the mitochondrial. Comparison of the several psbT gene sequences shows that there has been a four-nucleotide deletion in the two codons for 13 TL 14, resulting in a codon for Lys and a single nucleotide insertion in the codon for I 17 (Fig. 9B). Replacing the pea-specific KELV sequence with the conserved TLGII sequence results in a calculated mass of Da.
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Structure and Gene Content of Nephroselmis cpDNA. The NephroselmiscpDNA sequence assembles as a circle ofbp, with an overall A + T content of % (Fig.
(Fig.1). 1).Two identical copies of a large sequence (46, bp) containing the rRNA operon are present in inverted orientation and are separated from one another by LSC (92, bp) and SSC (16, bp) Cited by: Nucleotide sequence of two chloroplast genes from a Chlorella-like green alga: the large subunit of Ribulose-1,5-bisphosphate carboxylase/oxygenase and ribosomal protein S14Author: Sean M.
Amberg. Wakasugi T, Nagai T, Kapoor M, Sugita M, Ito M, Ito S, Tsudzuki J, Nakashima K, Tsudzuki T, Suzuki Y, Hamada A, Ohta T, Inamura A, Yoshinaga K, Sugiura M. Complete nucleotide sequence of the chloroplast genome from the green alga Chlorella vulgaris: the existence of genes possibly involved in chloroplast division.
Proc Natl Acad Sci by: Nucleotide sequence of the chloroplast 16S rRNA gene from the unicellular green alga Chlorella ellipsoidea Article (PDF Available) in Nucleic Acids Research 16(20) November with 30 Author: Takashi Yamada.
Nucleotide sequence of the chloroplast 16S rRNA gene from the unicellular green alga Chlorella ellipsoidea. T Yamada Mitsubishi Kasei Institute of Life Sciences, Tokyo, by: 6. The complete nucleotide sequence of the chloroplast genome (, bp) from the unicellular green alga Chlorella vulgaris C has been determined.
The genome contains no large inverted repeat and has one copy of rRNA gene cluster consisting of 16S, 23S, and 5S rRNA genes. Results. The ,bp Leptosira chloroplast genome resembles the ,bp Chlorella genome in lacking a large inverted repeat (IR) but differs greatly in gene of the conserved genes present in Chlorella cpDNA are missing from the Leptosira gene conserved genes, four introns and 11 free standing open reading frames (ORFs) account for % of the genome sequence.
The nucleotide sequences of four chloroplast tRNAs (methionine elongator, lysine, glycine, and arginine) from the siphonaceous green alga Codium fragile have been determined. These tRNAs have an unusually high A-U content compared to other chloroplast tRNAs and show varied, but in general only limited, sequence homology to the corresponding.
Genomic features. The Leptosira chloroplast genome sequence assembles as a circular molecule ofbp encoding a total of genes, not counting the intron ORF and the 11 free standing ORFs (Figure 1).All genes are present in single copy.
Table 1 compares the general features of Leptosira cpDNA with those reported for the eight other chlorophyte cpDNAs completely sequenced to date. Wakasugi T, Nagai M, Kapoor M et al. Complete nucleotide sequence of the chloroplast genome from the green alga Chlorella vulgaris: The existence of genes possibly involved in chloroplast division.
Proc Natl Acad Sci USA ; – PubMed CrossRef Google Scholar. Chloroplast DNAs are circular, and are typically ,–, base pairs long. They can have a contour length of around 30–60 micrometers, and have a mass of about 80– million daltons. Most chloroplasts have their entire chloroplast genome combined into a single large ring, though those of dinophyte algae are a notable exception—their genome is broken up into about forty small.
Abstract. Sequence determination of the chloroplast clpP gene from two distantly related Chlamydomonas species (C. reinhardtii and C. eugametos) revealed the presence of translated large insertion sequences (IS1 and IS2) that divide the clpP gene into two or three sequence domains (SDs) and are not found in homologous genes in other organisms.
These insertion sequences do not. We report the nucleotide sequence of the chloroplast psbA gene encoding the 32 kilodalton protein of photosystem II from Chlamydomonas moewusii.
Like its land plant homologues, this green algal. The complete nucleotide sequence of the chloroplast genome (, bp) from the unicellular green alga Chlorella vulgaris C has been determined. The genome contains no large inverted repeat and has one copy of rRNA gene cluster consisting of 16S, 23S, and 5S rRNA genes.
It contains 31 tRNA genes, of which the tRNALeu(GAG) gene has not been found in land plant chloroplast DNAs. A site-specific endonuclease was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA-containing virus NYs The enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the first C.
It cleaves 5'-C m C-3' sequences but not 5'- m CC-3' sequences. The enzyme creates breaks in dsDNA whenever two 5'-CC-3' sequences on. plant taxa so far examined.
Comparisons of nucleotide sequence divergence among chloroplast genes reveals marked similarity across the plant kingdom and beyond to the cyanobacteria (blue-green algae).
Estimates of rates of nucleotide substitution indicate a synonymous rate of x lOmy substitutions per site per year. Noncoding regions also.
Nucleotide Sequence of the psbB Gene of Chlamydomonas reinhardtii Chloroplasts Article (PDF Available) in Plant physiology 98(4) May with 7 Reads How we measure 'reads'. Nucleotide sequence of two chloroplast genes from a Chlorella-like green alga: the large subunit of Ribulose-1,5-bisphosphate carboxylase/oxygenase and ribosomal protein S Abstract.
Three CF 1 and three CF 0 subunits of the chloroplast H +-ATP synthase are encoded on the chloroplast chloroplast atp genes are organized as two Operons in plants but not in the green alga, Chlamydomonas atpBE or β Operon shows a relatively simple organisation and transcription pattern, while the atpIHFA or α Operon is transcribed into a large variety of.
The chloroplast elongator methionine-accepting tRNA from the green alga Scenedesmus obliquus was purified and and its nucleotide sequence determined. The nucleotide sequence of a tobacco chloroplast S rRNA gene, including the spacer between it and the S rRNA gene, has been determined.
The S rRNA coding region is base-pairs long.Hall JD, Karol KG, McCourt RM, Delwiche CF () Phylogeny of the conjugating green algae based on chloroplast and mitochondrial nucleotide sequence data.
J Phycol – Google Scholar Handa S, Nakahara M, Tsubota H, Deguchi H, Masuda Y, Nakano T () Choricystis minor (Trebouxiophyceae, Chlorophyta) as a symbiont of several species.Abstract. Since the presence of DNA was first detected in the chloroplasts of Chlamydomonas reinhardtii (Sager and Ishida, ), there has been intensive study of molecular aspects of plastid DNA from various species of green cells.
Chloroplasts are photosynthetic organelles that have their own genetic system, separate from the nuclear genome, which also encodes various plastid proteins.